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Original Research Article | OPEN ACCESS

Methanol Extract of Polyopes lancifolius Suppresses Tumor Necrosis Factor-α-Induced Matrix Metalloproteinase-9 expression in T24 Bladder Carcinoma Cells

R.G.P.T Jayasooriya1, Taek-Jeong Nam2, Gi-Young Kim1 , Yung Hyun Choi3,4

1Laboratory of Immunobiology, Department of Marine Life Sciences, Jeju National University, Jeju 690-756; 2Department of Food Science and Biotechnology, Pukyong National University, Busan 608-737; 3Department of Biochemistry and Research Institute of Oriental Medicine, Dongeui University College of Oriental Medicine, Busan 614-054; 4Department of Biomaterial Control (BK21 program) and Blue-Bio Industry Regional Innovation Center, Dongeui University, Busan 614-714, Republic of Korea.

For correspondence:-  Gi-Young Kim   Email: immunkim@jejunu.ac.kr   Tel:+82647543427

Received: 12 October 2011        Accepted: 17 April 2012        Published: 15 June 2012

Citation: Jayasooriya R, Nam T, Kim G, Choi YH. Methanol Extract of Polyopes lancifolius Suppresses Tumor Necrosis Factor-α-Induced Matrix Metalloproteinase-9 expression in T24 Bladder Carcinoma Cells. Trop J Pharm Res 2012; 11(3):405-412 doi: 10.4314/tjpr.v11i3.9

© 2012 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effects of the methanol extract of Polyopes lancifolius (MEPL) on the expression of matrix metalloproteinase-9 (MMP-9) and invasion in T24 human bladder carcinoma cells.
Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analyses were performed to assess the expression of MMP-9 and its regulatory proteins. MMP-9 activity was evaluated using zymography while matrigel infiltration was performed to assess T24 bladder carcinoma invasion. Electrophoretic mobility assay was used to investigate the nuclear factor-κB (NF-κB) activity.
Results: The expression and activity of MMP-9 were significantly increased in response to TNF-α, but MEPL suppressed TNF-α-induced MMP-9 expression and activity. MEPL also inhibited TNF-α-induced MMP-9 expression at the transcriptional level by blocking the activation of the NF-κB signaling pathway. Furthermore, the extract suppressed TNF-α-induced phosphorylation of IκBα and consequently sustained cytosolic p65 and p50 expression. Matrigel invasion assay showed that MEPL significantly reduced TNF-α-induced invasion of T24 bladder carcinoma cells.
Conclusion: Collectively, these data indicate that MEPL regulates TNF-α-induced MMP-9 expression by suppressing NF-κB activity.

Keywords: Polyopes lancifolius, Matrix metalloproteinase-9, Invasion; Nuclear factor-_4;B

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Thompson Reuters (ISI): 0.523 (2021)
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